Current Issue : July - September Volume : 2019 Issue Number : 3 Articles : 5 Articles
The use of morphine applied topically to painful wounds has potential advantages, such as\ndose reduction, fewer side effects and compound formulations, have been proposed for this purpose.\nGiven the potential high impact of drug product quality on a patientâ??s health, the aim of the present\nstudy was to develop two stable sterile hydrogels containing morphine hydrochloride, intended\nfor topical application on painful wounds. Two carboxymethylcellulose sodium-based hydrogels\nwere prepared containing 0.125% w/w (F1-MH semi-solid formulation) and 1.0% w/w (F2-MH\nfluid formulation) morphine hydrochloride (MH), respectively. Studies included a risk assessment\napproach for definition of the quality target product profile (QTPP) and assessment of critical\nquality attributes (CQA) of the hydrogels to support product quality and safety. Safe, odourless,\nyellowish, translucent and homogeneous gels were obtained, with suitable microbiological and\npharmaceutical characteristics. The active substance concentration was adapted according to the\ncharacteristics of the dose-metering device. Release profiles were investigated using Franz diffusion\ncells, and characterised by different kinetic models. Increasing gel viscosity prolonged drug release,\nwith rates ofâ?¦..................
The major challenge in the therapeutic applicability of oligonucleotide-based drugs is the\ndevelopment of efficient and safe delivery systems. The carriers should be non-toxic and stable\nin vivo, but interact with the target cells and release the loaded oligonucleotides intracellularly.\nWe approached this challenge by developing a light-triggered liposomal delivery system for\noligonucleotides based on a non-cationic and thermosensitive liposome with indocyanine green\n(ICG) as photosensitizer. The liposomes had efficient release properties, as 90% of the encapsulated\noligonucleotides were released after 1-minute light exposure. Cell studies using an enhanced green\nfluorescent protein (EGFP)-based splicing assay with HeLa cells showed light-activated transfection\nwith up to 70%-80% efficacy. Moreover, free ICG and oligonucleotides in solution transfected cells\nupon light induction with similar efficacy as the liposomal system. The light-triggered delivery\ninduced moderate cytotoxicity (25%-35% reduction in cell viability) 1-2 days after transfection, but\nthe cell growth returned to control levels in 4 days. In conclusion, the ICG-based light-triggered\ndelivery is a promising method for oligonucleotides, and it can be used as a platform for further\noptimization and development....
Self-assembled peptide hydrogels have emerged in recent years as the new paradigm in\nbiomaterials research. We have contributed to this field the development of hydrogels based on\ndehydrodipeptides N-capped with naproxen.The dehydrodipeptide hydrogels can be loaded with\ndrugs, thus being potential nanocarriers for drug delivery. In this work novel dehydrodipeptides\ncontaining tyrosine and aspartic acid amino acid residues N-capped with naproxen and C-terminal\ndehydrophenylalanine were prepared and characterized. Superparamagnetic iron oxide nanoparticles\n(SPIONs) were incorporated into the dehydrodipeptide-based hydrogels and their effect on the\nself-assembly, structure and rheological and magnetic properties of the hydrogels was studied.\nMagnetic hydrogels, with incorporated SPIONs, displayed concentration-dependent T2-MRI contrast\nenhancement. Moreover, upon magnetic excitation (alternating magnetic field -AMF-) the SPIONs\nwere able to generate a significant amount of heat. Hence, magnetic hyperthermia can be used\nas a remote trigger for release of drug cargos and SPIONs incorporated into the self-assembled\ndehydrodipeptide hydrogels....
Delivering an effective drug load to the posterior section of the ocular tissues, while using a\nnon-invasive technique, has always been a challenge. In this regard, the goal of the present study was\nto develop sustained release triamcinolone acetonide (TA) loaded polymeric matrix films for ocular\ndelivery. The TA-films were prepared in two different polymer matrices, with drug loadings of 10%\nand 20% w/w, and they were evaluated for ocular distribution in vivo in a conscious rabbit model.\nA 4%w/v TA suspension (TA-C) was used as a control for in vitro and in vivo studies. The TA-films,\nprepared with melt-cast technology, used polyethylene oxide (PEO) and Soluplus® as the polymer\nmatrix. The films were evaluated with respect to assay, content uniformity, excipient interaction, and\npermeability across isolated rabbit sclera. The distribution of TA in the ocular tissues, post topical\nadministration, was determined in New Zealand male albino rabbits as a function of dose, and was\ncompared against TA-C. The assay of the 10% and 20% w/w film was in the range from 70â??79% and\n92â??94% for the Soluplus® and PEO films, respectively, and content uniformity was in the range of\n95â??103% for both the films. The assay of the TA from Soluplus® films was less compared with the\nPEO films and showed an interaction with TA, as revealed by Differential Scanning Calorimetry\n(DSC). Hence, Soluplus® films were not selected for further studies. No interaction was observed\nbetween the drug and PEO polymer matrix. The enhancement of trans-scleral flux and permeability\nof TA was about 1.16 and 1.33-folds, respectively, from the 10% w/w PEO and 3.5 and 2.12-folds,\nrespectively, from the 20% w/w PEO films, as compared with TA-C formulations. The in vivo studies\ndemonstrate that significantly higher TA levels were observed in the anterior and posterior segments\nof the eye at the end of 6h with the PEO films. Therefore, the PEO based polymeric films were able to\ndeliver TA into the back of the eye efficiently and for prolonged periods....
A 70-year-old woman with Sjӧgren’s syndrome (SS) complained of generalized edema. Computed tomography showed thoracoabdominal fluid, suggesting serositis with SS. 35 mg/day of prednisolone as a monotherapy was ineffective. Moreover, hemoconcentration with hypoalbuminemia without inflammatory signs lead us to consider the systemic capillary leak syndrome(SCLS). Additional treatment with intravenous immunoglobulin (IVIG) and prednisolone dramatically decreased the thoracoabdominal fluid. However, when reducing the prednisolone dose, the thoracoabdominal fluid reincreased. Retreatment with IVIG without increasing the prednisolone dose was ineffective. However, additional prednisolone of 35 mg/day was effective, suggesting SCLS with SS might require combination therapy with IVIG and glucocorticoid....
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